EXPERIMENTAL AQUARIA

  • Experimental aquaria constitute essential PiE-UPV/EHU facilities where a wide range of experiment can be performed with the highest quality required. A plethora of experiments with marine algae, invertebrates and vertebrates can be carried out with highest quality standards.
  • PiE-UPV/EHU, as partner of EMBRC fulfills the compromise with the ethics and animal healthcare in experimentation regulations.
  • Facilties are modular, which allows a perfect design and the development of any experiment and especially useful in toxicological experimentation.
  • All experimental aquaria facilities are provided with independently regulated photoperiod and temperature, high quality seawater continuous supply (up to 300000 L/day) and air supply.
  • All the facilities are metal free, both outside and inside instrumentation.
  • Equipped with its own depuration plant organized in three depurations lines (organic material, hydrocarbons, metals) covering a huge range of contaminants that can be employed. Besides, specific filtration units could be installed for specific experiments if it is required.
  • Algae and plankton culture room to produce food for experimental fish and invertebrates.
  • The unit integrates a large dissection room and 10 different experimentation rooms.
  • Recently, new and modern equipment has been incorporated to improve the sea water quality and control:
    • a marine water temperature control system based in titanium plate interchanger allows the regulation of temperature in flow-through systems (12 m3/h; 14-22 ºC).
    • an advanced water quality monitoring platform with sensors (pH, conductivity, salinity, oxygen, turbidity, chlorophyll levels) and a data logger for continuous recording.
  • More than 20 experiments are conducted every year, including basic biology and toxicological experimentation with diversity of species such as algae, mollusks, crustaceans and fish. For instance:
    • Toxicological and growth experiments have been performed with marine mollucs such as periwinkles, oysters and mussels.
    • Toxicological experiments have been performed with sole, Solea sp., exposed to model contaminants in sediments and waterborne.
    • Sea bass was exposed to mixtures of emergent contaminants in open flow system using passive dosers in order to investigate changes at chemical, metabolomical and bio/histochemical levels in the sea bass.
    • Experiments carried out to investigate changes in the behavior patterns of sea bass fed Hg contaminated food, using under-water camera records to analyse the entropy of fish movement.
    • Sea urchin is used as model experimental species for biomedical (e.g., membrane lipids of sea urchin embryos as models for cancer research), as well as for toxicological assays.

Experimental aquaria facilities and equipment:

  • Field Equipment: sampling and caging devices; equipped mobile laboratory; 4x4 large vehicle, zodiac
  • Quarantine Aquaria Room
  • Toxic Algae Aquarium
  • PL2 Aquarium Room with PL3 Biosafety Cabinet
  • Invertebrate Nursery
  • Fish Nursery
  • Fish Embryo Toxicity Testing Unit
  • Microscale Toxicity Testing Aquarium
  • Low Capacity Aquaria (10-15 units of 500 l)
  • Medium capacity aquaria (10-15 units of 1000-1500 l)
  • High capacity aquaria (4-8 units of 2500-4000 l)
  • Outdoor mesocosms (2 units of 20000 l + 2 units of 10000)
  • Fully Equipped Dissection Room