TECNICAS_UTILIZADAS

Available techniques

  • Brain collection for psychiatric diseases and addiction disorders.
  • Biochemical and functional in vitro techniques for the study of neurotransmitter receptors (ligand binding, scintillation proximity assays (SPA), intra-cellular signalling responses, etc.).
  • Protein assessment techniques (western-blot, immunohistochemistry, ELISA, etc.) and metabolites.
  • Analysis of mRNA integrity (RIN number determination) and expression by quantitative RT-PCR.
  • Stereotactic surgery in rat and mouse.
  • Cerebral microdialysis in awake animal.
  • Electrochemical HPLC detection of monoamines and aminoacids.
  • Clinical microdialysis in human brain.
  • Ischemia and reperfusion techniques in rat brain.
  • Behavioural animal models of neuropsychiatric disorders and response to psychopharmacological treatments: locomotor activity, anxiety, cognitive impairment, social interaction, stress, PPI, etc.
  • Cell culture of olfactory neuroepithelium and other cell types from patients.