GENERAL GENOMICS SERVICE - GENE EXPRESSION UNIT

At the Genomics Laboratory that the Gene Expression Unit shares with the Genomics and Proteomics Sequencing and Genotyping Service, we have at our disposal high-performance equipment and state-of-the-art genomic technology for analysing global gene expression and for carrying out other analyses on a genomic scale, such as the study of chromosomal aberrations, transcriptional regulation, DNA methylation and micro RNAs. Below is a list of the most important equipment:

1. Precellys 24 Homogenizer for processing the sample (RNA extraction from tissues, blood and cell cultures).
2. Nucleic Acid bioanalyzer, spectrophotometer and fluorimeter for quality control and quantification of the samples (DNA and RNA).
3. Real time PCR system with 96 well-plate fast block, 384 well-plate block and block for the TaqMan Low-Density Arrays.
4. BioMark HD nanofluidic real time PCR System (Fluidigm Corporation)
5. Microarray hybridization platform from Agilent Technologies: Hybridization oven and microarray rotor, and G2565CA Microarray Scanner.

TISSUE HOMOGENIZER

Precellys 24

HIGH-PRECISION QUANTIFICATION

QUALITY CONTROL ANALYSIS OF TOTAL RNA, MESSENGER RNA AND MICRO-RNAS AND MIGRATION OF AMPLIFIED DNA FRAGMENTS AND DIGESTION

          
Agilent 2100 Bioanalyzer

7900HT FAST REAL-TIME PCR SYSTEM

BIOMARK HD NANOFLUIDIC REAL TIME PCR SYSTEM (FLUIDIGM CORPORATION)

MICROARRAY HYBRIDIZATION OVEN MADE BY agilent TECHNOLOGIES

G2565CA MICROARRAY SCANNER MADE BY AGILENT TECHNOLOGIES

AUTOMATIC PREPARATION ELECTROPHORESIS SYSTEM IN GEL PIPPIN PREP (SAGE SCIENCE)

Automatic system for the selection of DNA fragments of interest from agarose gels, in the ranges of 100 bp to 1500 bp. Its main application is the preparation of NGS libraries, especially libraries of microRNAs and small RNAs (small RNAs).