Abstract

Accumulation of abaxial anthocyanins is an intriguing leaf trait particularly common among deeply-shaded understorey plants of tropical and temperate forests whose ecological significance is still not properly understood. To shed light on it, possible ecophysiological roles of abaxial anthocyanins were tested in the perennial understorey herb of temperate deciduous forests Saxifraga hirsuta, chosen as model species due to the co-existence of green and anthocyanic leaves and the presence of an easily removable lower anthocyanic epidermis. Anthocyanins accumulated during autumn, which temporally matched with overstorey leaf fall. Patterns of development of abaxial anthocyanins and direct measurements of photochemical efficiency under monochromatic lights were not consistent with a photoprotective hypothesis. Enhancement of light capture seemed also unlikely since the backscattering of red light towards the lower mesophyll was negligible. Seed germination was similar under acyanic and anthocyanic leaves. A relevant consequence of abaxial anthocyanins was the dramatic reduction of light transmission through the leaf. Dark environment generated underneath the Saxifraga canopy was enhanced by the horizontal repositioning of leaves, which occurs in parallel to reddening. This might play a role in the biotic interactions by inhibiting vital processes of competitors, which maybe of especial importance in spring before the overstorey leaves sprout.

Abstract

The Ecological Risk Assessment (ERA) is a reliable tool for communicating risk to decision makers in a comprehensive and scientific evidence-based way. In this work, a site-specific ERA methodology based on the TRIAD approach was applied to contaminated soil surrounding a closed non-sealed landfill, as a case study to implement and validate such ERA methodology in the Basque Country (northern Spain). Initially, the procedure consisted of the application of a Parameter Selection Module aimed at selecting the most suitable parameters for the specific characteristics of the landfill contaminated soil, taking into consideration the envisioned land use, intended ecosystem services and nature of contaminants. Afterwards, the selected parameters were determined in soil samples collected from two sampling points located downstream of the abovementioned landfill. The results from these tests were normalized to make them comparable and integrable in a risk index. Then, risk assessment criteria were developed and applied to the two landfill contaminated soil samples. Although the lack of a proper control soil was evidenced, a natural land use was approved by the ERA (at Tier 2) for the two landfill contaminated soils. However, the existence of a potential future risk resulting from a hypothetical soil acidification must be considered.

Abstract

The molecular response of Pseudomonas fluorescens cells exposed to a mixture of heavy metals remains largely unknown. Here, we studied the temporal changes in the early gene expression of P. fluorescens cells exposed to three doses of a polymetallic solution over two exposure times, through the application of a customized cDNA microarray. At the lowest metal dose (MD/4), we observed a repression of the Hsp70 chaperone system, MATE and MFS transporters, TonB membrane transporter and histidine kinases, together with an overexpression of metal transport (ChaC, CopC), chemotaxis and glutamine synthetase genes. At the intermediate metal dose (MD), several amino acid transporters, a response regulator (CheY), a TonB-dependent receptor and the mutT DNA repair gene were repressed; by contrast, an overexpression of genes associated with the antioxidative stress system and the transport of chelates and sulfur was observed. Finally, at the highest metal dose (4MD), a repression of genes encoding metal ion transporters, drug resistance and alginate biosynthesis was found, together with an overexpression of genes encoding antioxidative proteins, membrane transporters, ribosomal proteins, chaperones and proteases. It was concluded that P. fluorescens cells showed, over exposure time, a highly complex molecular response when exposed to a polymetallic solution, involving mechanisms related with chemotaxis, signal transmission, membrane transport, cellular redox state, and the regulation of transcription and ribosomal activity.