GENERAL GENOMICS SERVICE SEQUENCING AND GENOTYPING UNIT

Introduction

Numerous research groups are currently involved in projects that incorporate genetic studies. However, the high cost of the equipment and the specialist nature of the techniques makes it difficult for each research group to be able to ensure that the equipment necessary for expression analyses and massive genotyping is at their disposal.

The Sequencing and Genotyping Unit of the University of the Basque Country, UPV/EHU, has been set up in order to provide both the knowledge and equipment necessary to researchers from the Basque Network of Science, Technology and Innovation, biocompanies from the milieu and to public and private institutions throughout Spain and overseas, in activities related to genetic analysis.  To this end, it has qualified personnel at its disposal together with state-of-the-art techniques and equipment in automatic DNA sequencing and genotyping, and a recently-created laboratory designed in accordance with the recommendations of laboratory good practices.

The wide experience and importance of this university service is evidenced by the high number of users that are currently using it, and by the large volume of work that is being administered in this section.
This unit carries out a series of services such as:

  • extraction, quantification and normalization of genomic and mitochondrial DNA from different tissues.
  • amplification, purification and quantification reactions of PCR and plasmic DNA products. 
  • DNA sequencing,
  • genotyping of different DNA markers: RFLPs, STRs or microsatellites, SNPs, CNVs, etc.

The quality of Sequencing and Genotyping is backed up by international genetic analysis societies via the participation of the UPV/EHU Service in numerous quality controls and authorisations for domestic animal genotyping (Comparation test of the Internatinal Society of Animal Genetic; ISAG) and genotyping in humans  (officially approved by the Spanish and Portuguese Group of the International Society of Forensic Genetics; GEP-ISFG)