Info_Biomedicina_Genomica_secuenciacion_servicios_concretar_genotipo

GENOTYPE of genomic DNA fragments: STRs, SNPs, indels, ...

  • STRs, indels and other types of genomic DNA fragments (STR Code):
    • Standard amplification reactions (PCR, multiplex PCR) and Whole Genome Amplification (WGA). (PCR code).
    • Capillary electrophoresis -> Fragment genotyping, STRs and indels (see document).
      • Determination of size of fluorescently labeled DNA amplified (fragments of genomic DNA).
      • Microsatellite multiplex genotyping (STRs): simultaneous detection of up to 5 colors.

 

  • SNPs and Indels (SNP Code):
    • BioMark HD System - Fluidigm (multiplex of 12-48-96 SNPs vs samples) (see document).
    • TaqMan - single assays (1 SNP per reaction, RT-PCR analysis in equipment CFX96) (see document).
    • HRMA (High Resolution Melt Analysis) in equipment CFX96
    • SNaPshot (1-20 SNPs by multiple PCR and capillary migration detection) (see document).