Behaviour and ultrastructure of human bone marrow-derived mesenchymal stem cells immobilised in alginate-poly-l-lysine-alginate microcapsules.
- Autoría:
- Attia N, Santos E, Abdelmouty H, Arafa S, Zohdy N, Hernandez RM, Orive G, Pedraz JL.
- Año:
- 2014
- Revista:
- Journal of Microencapsulation
- Volumen:
- 31(6)
- Página de inicio - Página de fin:
- 579 - 589
- ISBN/ISSN:
- 0265-2048
- Descripción:
-
Abstract
CONTEXT:
Human bone marrow mesenchymal stem cells (hBM-MSCs) show a great promise for the treatment of a variety of diseases. Despite the previous trials to encapsulate hBM-MSCs in alginate-poly-l-lysine-alginate (APA) systems, the various changes that follow immobilisation have not been ascertained yet.
OBJECTIVE:
Determine the various consequences derived from entrapment on cell behaviour, putting special emphasis on the ultrastructure.
METHODS:
hBM-MSCs were immobilised in APA microcapsules to further characterise their viability, metabolic activity, proliferation, VEGF-secretability, and morphology.
RESULTS:
The VEGF produced by monolayer hBM-MSCs increased significantly 1 d post-encapsulation, and was maintained for at least 4 weeks. TEM imaging of cells revealed well preserved ultrastructure indicating protein synthesis and high metabolic activity.
CONCLUSION:
Although APA microencapsulation did not support 100% of fully viable hBM-MSCs for long-term cultures, it was conceived to enhance both VEGF secretion and metabolic activity while not losing their stemness characteristics.