Nanoparticles may assemble in different ways, in the form of wires, 3D crystals, or mono and multilayers. The assembly may be controlled by the chemical composition of the organic layer coating the particle, and it may be influenced by evaporation rate and flow forces. Additionally it has been shown that degas-driven flow microfluidics, may be used to create high resolution multicomponent patterns on a surface. We have combined both processes and we obtained a methodology to create multicomponent patterns of nanoparticles assemblies with high resolution on a glass surface. Additionally we have shown the functionality of such assemblies for SERS applications.