The tesis describes the development of point-of-care (PoC) diagnostic devices designed to provide rapid, low-cost, and decentralized testing without the need for specialized laboratories. These systems are guided by the World Health Organization’s REASSURED criteria, which ensure accessibility, ease of use, and high analytical performance, particularly in resource-limited settings. In this context, electrochemiluminescence (ECL) emerges as a highly promising technique due to its sensitivity and compatibility with miniaturized systems. Additionally, screen printing is highlighted as a key fabrication method, enabling the production of low-cost, reproducible, and customizable sensors, and facilitating the integration of functional materials into portable diagnostic platforms.

The experimental work focuses on several strategies to enhance device performance. Laser treatment of screen-printed electrodes significantly improves their electrochemical properties and sensing capabilities. A self-powered PoC device is also developed, using wireless energy transfer via NFC from a smartphone to operate an ECL sensor, eliminating the need for external power sources. Furthermore, the study explores signal amplification in the L-012 ECL system under specific electrochemical conditions, achieving substantial increases in signal intensity, particularly with microelectrodes. Overall, the thesis demonstrates how combining advanced fabrication techniques, signal optimization, and wireless power harvesting can lead to more sensitive, accessible, and practical PoC diagnostic devices.