Equipment of the month: Q Exactive HF-X mass spectrometer for proteomic purposes

The Proteomics Core Facility at the University of the Basque Country has installed a new Q Exactive HF-X mass spectrometer coupled to an EASY nLC-1200 nanoUPLC system.

This equipment is a state of the art hybrid quadrupole-Orbitrap mass spectrometer that combines high capacity transfer tube, high performace quadrupole analyzer and high resolution accurate mass (HRAM) ultra-high-field Orbitrap mass analyzer to provide excellent achievement in both, targeted and untargeted analysis. It supports multiple acquisition modes, such as DDA (Data Dependent Acquisition), SIM (Selected Ion Monitoring), PRM (Parallel Reaction Monitoring) and DIA (Data Independent Acquisition). A detailed description of the instrument can be found in the publications 1 and 2.

This instrument can be used in most of the botton-up proteomics applications. The Q Exactive HF-X can successfully perform botton-up type of analysis with low amount and/or highly complex samples.

• Botton-up protein identification and quantification continues to be the basis for much of the proteomic research.
• Targeted quantification of known protein targets.
• Post Translational Modification analysis in order to determine the specific modification site and its relative quantification.

The instrument has already been used to identify and relatively quantify proteins in samples as diverse as human exosomes and plant roots. It has also proven very useful for the analysis of ubiquitin modified proteins in Drosophila melanogaster and human cells. Publications describing the use of Q Exactive HF instruments for different proteomic applications are 3, 4, 5, 6, and 7.

• Center for Animal Biotechnology "Maria Goyri"
• Laboratory 0.02
• Campus of Bizkaia.
• 48940, Leioa.

• Kerman Aloria
• kerman.aloria@ehu.eus
• 946013278