The main objective of the MISCA project is to develop innovative platforms for studying cellular secretion in physiologically relevant 2D and 3D microenvironments, with real-time monitoring capabilities and without the need for markers. Microfluidic systems, sensors based on structural signalswitching aptamers (SSSA), and functionalized surfaces will be combined to provide highly sensitive and precise tools for investigating cellular processes in complex pathological models. This platform allows continuous and marker-free monitoring, preserving cell viability and respecting the natural dynamics of the system, thus overcoming the limitations of traditional methods that require invasive techniques or fluorescent labels. Additionally, advanced 2D and 3D cellular patterns that more accurately simulate in vivo physiological environments are incorporated, offering a more representative perspective of cellular processes. This is a versatile and precise tool that will enable the study of subtle cellular events that conventional methods fail to detect. The MISCA project builds on previous work carried out by the UPV/EHU Microfluidics Cluster, which has developed advanced techniques like the Printand- Vacuum Lithography (PnV Litho) technique for creating 2D and 3D cellular patterns enabling the placement of sensors in immediate proximity to the cells. Furthermore, the team has demonstrated its ability to detect proteins in culture media using structural signal-switching aptamers (SSSA). Extensive work has also been conducted on the design and fabrication of efficient microfluidic chips. All of this provides a solid foundation for the current project. The research team includes Assoc. Prof. Fernando Benito and Prof. Lourdes Basabe as principal leaders, along with Prof. Idoia Postigo, an immunology specialist. Additionally, the team is complemented by electronic engineers, pharmacists, and PhD students, who contribute specialized knowledge in biotechnology and health sciences. The MISCA platform will employ 2D and 3D cellular microenvironments designed using Print-and-Vacuum Lithography (PnV Litho), enabling precise control of experimental conditions. The aptamer-based sensors, built on streptavidin-functionalized beads, will allow dynamic detection of cytokine secretion in cellular cultures. The system will be validated using Jurkat model cells, modified to overexpress the SLAMF3 receptor and exposed to palmitic acid to simulate inflammatory conditions associated with obesity. Additionally, fluorescence microscopy will be used to characterize secretion events in real time and validate the sensors' functionality. The UPV/EHU Microfluidics Cluster has all the necessary infrastructure, including laboratories for device fabrication (photolithography, 3D printing, profilometry), as well as equipment for cell culture and advanced microscopy. The team also has access to all relevant UPV/EHU infrastructure, such as scanning electron microscopy.